0 Recommendations

Fixing methods for MGIT culture smear

By Ranjan Perera | 09 Jul, 2019

In our laboratory when we make smears from MGIT positive cultures, they get washed off completely during staining. We use a heat block and fix for two hours at 75 degrees. But it does not seem to help. We have tried formal milk before but it did not work as well. What could be the problem? (We clean the slices prior to use to make sure no oil residuals are present).

What other fixatives do you use in your laboratories?

Please help.

Replies

 

Mengistu Tadesse Replied at 10:16 PM, 9 Jul 2019

--

Kishore Reddy VC Replied at 12:27 AM, 10 Jul 2019

Hi please try with 2% to 5% Bovine albumin as a fixative

--

*Dr. Kishore Reddy VC.** PhD*
* Microbiologist*
International Center for Excellence in Laboratory Training-ICELT

This Community is Archived.

This community is no longer active as of December 2018. Thanks to those who posted here and made this information available to others visiting the site.