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Line probe assays (LPAs) types

By Ibtihal omer | 17 Jul, 2018

Dear experts, could you please give me brief information to differentiate between:
1. Genotype MTBDR assay
2. GenoType MTBDRsl VER 1.0
3. GenoType MTBDRsl VER 2.0
4. The GenoType MTBDRplus assay

Replies

 

Ranjan Perera Replied at 10:28 AM, 17 Jul 2018

MTB DR SL version one
tests for FLQ, Injectables and Ethambutol
Does not differentiate resistance among the second line injectables
only include gyrA for fluoroquinolone resistance
less sensitive and specific than the version 2

MTB DR SL
include gyrA and gyrB for fluoroquinolone resistance
ethambutol is excluded
included the eis promoter to detect low level kanamycin resistance
can differentiate the resistance to individual drugs
more sensitive than version 1
more specific than version 1

i do not know about MTBDR, is it an old version of the MTBDR plus?

Kishore Reddy VC Replied at 12:10 AM, 18 Jul 2018

Hi

1. Genotype MTBDR assay - To test and detect M.TB and its resistance to
Rifampicine only.

2. GenoType MTBDRsl VER 1.0 To test and detect M.TB and it resistance to
a)fluoroquinolones- *gyrA gene*, b)second line injectables/cycle peptides*-rrs
gene* & c) ethambutol-*emb *gene.

3. GenoType MTBDRsl VER 2.0 To test and detect M.TB and it resistance to
a)fluoroquinolones- *gyrA & gyrB gene*, b)second line injectables/cycle
peptides-*rrs gene* & c) Low level kanamycin -*eis gene.*

4. The GenoType MTBDRplus assay - To test and detect M.TB and its
resistance to Rifampicine- *RpoBgene * Isoniazid Resitance -* KatG & InhA
gene*

*with best regards*
*Kishore*


--

*Dr. Kishore Reddy VC.** PhD*
* Microbiologist*
International Center for Excellence in Laboratory Training-ICELT

Mengistu Tadesse Replied at 11:47 AM, 18 Jul 2018

Dear Ibtihal omer
<http://email.ghdonline.org/c/eJxlUMuKxCAQ_Jp4W2kfiV48LAzzG4MTO4kQNWiHMH-_BnZP...>
,

*GenoType MTBDRsl VER 2.0** is new method next to V-1 which is as commented
by[ **Kishore Reddy VC*
<http://email.ghdonline.org/c/eJxFT1FrgzAY_DXmrUVNou4hD52ljK0rbKOj3YtY86mhmoTk...>
* ]
WHO-recommended rapid Molecular qualitative in vitro test for the
identification of the Mycobacterium tuberculosis complex and its
resistance to fluoroquinolones ((FLQ[ofloxacin and moxifloxacin]) and
aminoglycosides/cyclic peptides (AG/CP); injectable antibiotics such as
kanamycin, amikacin, capreomycin, and viomycin) gyrA gene and gyrB codes
for A and B-subunit of the DNA gyrase respectively gyrA Its coded from
codon 85 to 97Mutation occurs at codon 88, 90, 91, and 94gyrB mutation
occur at codon 538, 540 Fluoroquinolones (ofloxacin and moxifloxacin)
Inhibits DNA qyrase which allows ease of the DNA to be unwound during
replication.* *rrs gene encodes for cell wall development rrs gene is
300bpIts coded at codon 1401 to 1484.* *Eis gene encodes for low level
Kanamycin Mutation occur at codon, 2, 10,12, 14, 37 .
from NALC/NaOH processed smear-positive or -negative sputum specimens and
isolates of Liquid or solid culture. please see
the attached policy statement for more information .*

*The GenoType MTBDRplus assay not MTBDR is WHO-recommended
rapid Molecular qualitative in vitro test for the identification of
the Mycobacterium tuberculosis complex and its resistance to ** Rifampicin
and Isoniazid Resistance to Rifampicin is by the detection of mutations
inthe rpoB gene. Resistance to Isoniazid is by the detection of the most
common mutations in the katG gene and inhA gene . from NALC/NaOH processed
smear-positive sputum specimens and isolates of Liquid or solid culture.
please see the attached policy statement for more information .*

Doris Hillemann Replied at 8:37 AM, 19 Jul 2018

Hi,

Genotype MTBDR assay - To test and detect M.TB and its resistance to
Rifampicine only. Test was also for Isoniazid resistance, but only katG

Doris

from the NRZ in Germany


--
Dr. Doris Hillemann
Wissenschaftliche Mitarbeiterin

Forschungszentrum Borstel
Leibniz-Zentrum für Medizin und Biowissenschaften/Medizinische Klinik
Nationales Referenzzentrum für Mykobakterien
Parkallee 18
23845 Borstel
Tel. *49 (0)4537 188 7610
Fax *49 (0)4537 188 3110

www.fz-borstel.de

Das Forschungszentrum Borstel ist eine rechtsfähige Stiftung des bürgerlichen Rechts.
Vertretungsberechtigte Personen sind: Prof. Dr. Stefan Ehlers (Zentrumsdirektor),
Prof. Dr. Susanne Krauss-Etschmann (Direktorin), Prof. Dr. Ulrich Schaible (Direktor),

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